LOCUS       sequence-146799-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of wild-type or mutant cDNA fragments 
            (mutation reporter) or complete cDNAs in frame with superfolderGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  TITLE     pCS2+MCS-P2A-sfGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS2+MCS-P2A-sfGFP
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    353..374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(389..394,395..412,413..419)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    427..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     432..454
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     451..467
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     451..467
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     486..506
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        488..506
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    619..753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(656..675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     710..729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(826..846)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      241 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      301 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      361 gttagctcac tcattaggca ccccaggctt tacactttat gctcccggct cgtatgttgt
      421 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      481 agcgcgcaat taaccctcac taaagggaac aaaagctggg taccgggccc aatgcattgg
      541 cgccgcggcc gcgaattaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      601 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      721 aaactcatca atgtatctta tcatgtctgg atctacgtaa tacgactcac tatagttcta
      781 gattatttgt agagctcatc catgccatgt gtaatcccag cagcagttac aaactcaaga
      841 aggaccatgt ggtcacgctt ttcgttggga tctttcgaca ggacagattg tgtcgacagg
      901 taatggttgt ctggtaaa
//
