LOCUS       sequence-146804-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCP-3XGFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-EFS-PCP-3XGFPnls
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atccagaggt tgattatcta gattacactt tccgcttttt ctttggctcg agtttgtaca
       61 gttcatccat accatgcgtg atgcccgctg cggttacgaa ctccagcaga accatatgat
      121 cgcgtttctc gttcggatct ttagacagaa cgctttgcgt gctcagatag tgattgtctg
      181 gcagcagaac aggaccatca ccgattggag tgttttgctg gtagtgatca gccagctgca
      241 cgctgccatc ctccacgttg tggcgaattt taaaattcgc tttaatgcca tttttttgtt
      301 tatcggcggt gatgtaaaca ttgtggctgt taaaattgta ttccagctta tggcccagga
      361 tattgccgtc ttctttaaag tcaatgcctt tcagctcaat gcggtttacc agggtatcgc
      421 cttcaaattt cacttccgca cgcgttttgt acgtgccgtc atccttaaag gaaatcgtgc
      481 gttcctgcac atagccttcc ggcatggcgg acttgaagaa gtcatgctgc ttcatatggt
      541 ccggataacg agcaaagcac tgaacaccat aagtcagcgt cgttaccaga gtcggccaag
      601 gtaccggcag tttaccagta gtacagatga acttcagcgt cagtttacca ttagttgcgt
      661 caccttcacc ctcgccacgc acggaaaact tatgaccgtt gacatcacca tccagttcca
      721 ccaga
//
