LOCUS       sequence-146806-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N22p-3XRFPnls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pHAGE-EFS-N22p-3XRFPnls
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..32)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(314..333)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     583..601
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tatctagatt acactttccg ctttttcttt ggctcgagct tgtacagctc gtccatgccg
       61 ccggtggagt ggcggccctc agcgcgttcg tactgttcca cgatggtgta gtcctcgttg
      121 tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc
      181 ttggccttgt aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc
      241 ctctgcttga tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc
      301 tcccagccca tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc
      361 agcttcacct tgtagatgaa ctcgccgtcc tgcagtgagg agtcctgggt cacggtcacc
      421 acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc
      481 ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac
      541 tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg
      601 gcggtctggg tgccctcgta ggggcgg
//
