LOCUS       sequence-146812-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for miRNA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, 
            Pantel K, Schwarzenbach H
  TITLE     Increased serum levels of circulating exosomal microRNA-373 in 
            receptor-negative breast cancer patients.
  JOURNAL   Oncotarget. 2014 Oct 30;5(20):9650-63.
  PUBMED    25333260
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Oncotarget."; date: "2014-10-30"; volume: "5(20)"; pages: "9650-63"
COMMENT     Sequence Label: 190_SMAD4 in  pmiRGlo
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(231..249)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    286..407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(297..316)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     351..370
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(675..695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
       61 ggcggcaaga tcgccgtgta attctagttg tttaaacgag ctcgctagct ctaagccctt
      121 tgccatcaat gatcatatca attggcagtg actctagagt cgacctgcag gcatgcaagc
      181 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      241 ataactagca taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg
      301 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      361 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      421 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa
      481 acctctacaa atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga
      541 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc
      601 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg
      661 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      841 c
//
