LOCUS       sequence-146813-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgGFP-2 with blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: PXPR007 sgGFP-2
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..58)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(74..121)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tggggccggg gttctcctcc acgtcgccgg cctgcttcag caggctgaag ttggtggcgc
       61 cgctgccgct agctttcttt ttcttagctt gaccagcttt cttagtagca gcaggacgct
      121 tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg atgctctggt
      181 ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg tcgatggtgg
      241 tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac aggtggatga
      301 tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg gacagcactt
      361 tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc tgctcgatga
      421 tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca ttatcctcgg
      481 gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca tatttggagg
      541 gcagggccag ttcgtttccc ttctgcagtt cgccggcaga ggccagcatt ctcttccggc
      601 cgttttccag ctcgaacagg gagtacttag gcagcttgat gatcaggtcc tttttcactt
      661 ctttgtagcc cttggcttcc agaaagtcga tgggattctt ctcgaagctg cttctttcca
      721 tga
//
