LOCUS       sequence-146814-         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1-2XMS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-2XMS2
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     489..508
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
       61 gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag
      121 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
      181 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
      241 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg
      301 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
      361 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
      421 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
      481 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
      541 agtggctgat ctcagccacc gcgaaa
//
