LOCUS       sequence-146817-         396 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1-2XPP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-2XPP7
FEATURES             Location/Qualifiers
     source          1..396
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..367)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(164..183)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccggagcgac gccatatcgt ctgctccggt agctctcaaa cgggtcttcg tcctgcaggg
       61 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      121 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
      181 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg
      241 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
      301 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
      361 actgcatttc accagtccct gttctcgtca gcaaaa
//
