LOCUS       sequence-146820-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1-MS2-PP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-MS2-PP7
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(29..47)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     CDS             complement(85..390)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(187..206)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccttatttga acttgctagg ccctgcagac atgggtgatc ctcatgttgg cctagctctc
       61 aaacgggtct tcgtcctgca gggtttatat tccccagaac atcaggttaa tggcgttttt
      121 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
      181 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
      241 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
      301 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
      361 tcttttatag gtgtaaacct taaactgcat ttcaccagtc cctgttctcg tcagcaaaag
      421 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
      481 agcgttcggc acgcagacga cgggcttcat tctgca
//
