LOCUS       sequence-146823-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for 
            expression control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Paquet D, Kwart D, Chen A, Sproul A, Jacob S, Teo S, Olsen KM, Gregg
            A, Noggle S, Tessier-Lavigne M
  TITLE     Efficient introduction of specific homozygous and heterozygous 
            mutations using CRISPR/Cas9.
  JOURNAL   Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 
            2016 Apr 27.
  PUBMED    27120160
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17664"; journalName: "Nature"; date: "2016-05-5- 5"; 
            volume: "533"; issue: "7601"; pages: "125-9"
COMMENT     Sequence Label: pCas9-VRER_2A_GFP
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..58)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(74..94)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccattgggcc gggattctcc tccacgtcac cgcatgttag aagacttcct ctgccctctc
       61 cgccgccgga cctcaccttc ctcttcttct tggggtcagc cctgctgtct ccaccgagct
      121 gagagaggtc gattcttgtt tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt
      181 ccaggacctc ctttgtagag cggtactcct ttctgtctat ggtggtgtcg aagtacttga
      241 aggctgcagg cgcgcccaag ttggtcagag taaacaagtg gataatgttt tctgcctgct
      301 ccctgatggg cttatccctg tgcttattgt aagcagaaag caccttatcg aggttagcgt
      361 cggcgaggat cactcttttg gagaattcgc ttatttgctc gatgatctca tcaaggtagt
      421 gtttgtgttg ttccacgaac agctgcttct gctcattatc ttcgggagac cctttgagct
      481 tttcatagtg gctggccaga tacaagaaat taacgtattt agagggcagt gccagctcgt
      541 tacctttctg cagctcgcgc gcactagcga gcattcgttt ccggccgttt tcaagctcaa
      601 agagagagta cttgggaagc ttaatgatga ggtctttttt gacctcttta tatcctttcg
      661 cctcgagaaa gtcgatggg
//
