LOCUS       sequence-146830-         522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgCIC-1, puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pXPR_BRD003 sgCIC-1
FEATURES             Location/Qualifiers
     source          1..522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        123..198
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    254..371
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgcgcagggg ccccataccc
      121 cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      181 gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag
      241 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      301 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      361 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
      421 gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
      481 ccttctaggt cttgaaagga gtgggaattg gctccggtgc cc
//
