LOCUS       sequence-146834-         557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1-boxB-MS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-boxB-MS2
FEATURES             Location/Qualifiers
     source          1..557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..47
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             complement(80..385)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(182..201)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aacggactag ccttatttga acttgctagg gcccttcttc agggccctag ctctcaaacg
       61 ggtcttcgtc ctgcagggtt tatattcccc agaacatcag gttaatggcg tttttgatgt
      121 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg
      181 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
      241 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
      301 cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
      361 tataggtgta aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg
      421 ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt
      481 tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg
      541 acatcatata tgccttg
//
