LOCUS       sequence-146840-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , 
            Emission: 475 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     ECFP/Venus mitochondrial-targeted calcium biosensor
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 2MT-D2-CPV
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..153
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             175..249
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(277..279,280..282,283..795)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(322..343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(583..602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
      121 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttggggga tcccatgtcc
      181 gtcctgacgc cgctgctgct gcggggcttg acaggctcgg cccggcggct cccagtgccg
      241 cgcgccaaga tccattcgtt ggggaagctt gccaccatgg tgagcaaggg cgaggagctg
      301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaggttc
      361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
      481 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      541 atgcccgaag gctacgtcca ggagcgtacc atcttcttca aggacgacgg caactacaag
      601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
      721 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccca cttcaagatc
      781 cgccacaaca tcgaggacgg
//
