LOCUS       sequence-146850-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modular vector for secreted amylase reporter expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Oliveira Paiva AM, Friggen AH, Hossein-Javaheri S, Smits WK
  TITLE     The Signal Sequence of the Abundant Extracellular Metalloprotease 
            PPEP-1 Can Be Used to Secrete Synthetic Reporter Proteins in 
            Clostridium difficile.
  JOURNAL   ACS Synth Biol. 2016 Jun 23.
  PUBMED    27333161
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 23."
COMMENT     Sequence Label: pWKS1583
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatagagtat aattaaaata agcttgatcg tagcgttaac agatctgagc tcctgcagta
       61 aaggagaaaa ttttatgaga ccaagtaaaa aattattaat agctataata tcaatattct
      121 taataagttc agttccagta agtgcacatg cagatagtgc aaacaaaagt aatgaattaa
      181 cagcacctag tatcaaaagt ggaacaattc tacatgcttg gaactggtca tttaacactt
      241 tgaaacacaa tatgaaagat atacatgatg ctggatatac agcaatacag acttctccaa
      301 taaaccaggt aaaagaggga aatcaagggg ataaatcaat gtctaattgg tattggttat
      361 atcaaccaac atcatatcaa ataggaaata ggtatcttgg tactgaacaa gagtttaaag
      421 aaatgtgtgc tgcagctgaa gaatatggaa taaaggttat tgtagatgct gtcataaatc
      481 atacaacttc agactatgct gctatatcta acgaagtaaa gtctattcca aattggacac
      541 atggtaatac acagattaag aattggtctg atcgatggga tgtgacacaa aactctttac
      601 taggtcttta tgattggaat actcaaaata ctcaagtaca atcatatctt aaaagatttt
      661 tggacagggc tcttaacgat ggagcagatg gttttagatt cgatgcagca aaacacatag
      721 aattgccaga cgatggatca tatggtagtc agttttggcc aaacataaca aatactagtg
      781 ctgaatttca atatggtgag attttacaag attctgcaag tagagatgca gcatatgcga
      841 actatatgga tgttactgct agtaattacg gtcatagtat tagatcagct ctaaagaata
      901 ggaatttagg agtttctaat atctctcatt atgcctctga tgtttcagca gataaactag
      961 taacttgggt tgaaagccat gatacatatg caaacgacga tgaagaatct acatggatgt
     1021 ctgatgatga tataagatta ggttgggcag ttatagcatc ta
//
