LOCUS       sequence-146895-        9145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID positive control lentiviral plasmid. Expresses 
            Cbx1-V5-Dam..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pLgw Cbx1-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..9145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(485..1276,1277..1345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1108..1127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1346..1450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1525..1544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1716..2095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2097..2295
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2250..2270
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2313..2493
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2537..2662
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3155..3388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3573..3617
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4079..4317
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4264..4285
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    4370..4394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4986..5010)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5021..5062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     LTR             6427..6607
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6633..6650)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6639..6863
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6909..7337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6996..7015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7206..7227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7346..7366)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7351..7681
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7532..7667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7594..7613
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7729..7776
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7795..8169
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8299..8420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8336..8355)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8390..8409
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8469..8485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8469..8485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8482..8504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8493..8509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8517..8523,8524..8541,8542..8547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8562..8583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8700..8717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8871..9145,1..314))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8951..8970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
       61 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
      721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
      781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
      841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
      901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
      961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     1501 cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     1561 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     1621 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     1681 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     1741 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1801 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1861 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1921 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1981 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2041 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2101 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2161 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2221 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2281 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
     2341 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     2401 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     2461 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acctgaaagc
     2521 gaaagggaaa ccagagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2581 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2641 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2701 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2761 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2821 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2881 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2941 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3001 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3061 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3121 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3181 gagcagcagg aagcactatg ggcgcagcct caatgacgct gacggtacag gccagacaat
     3241 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3301 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3361 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3421 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatctgga
     3481 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3541 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3601 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3661 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3721 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3781 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     3841 gatccattcg attagtgaac ggatcccccg ggctgcagga attcagatct cggccgcata
     3901 ttaagtgcat tgttctcgat accgctaagt gcattgttct cgttagctcg atggacaagt
     3961 gcattgttct cttgctgaaa gctcgatgga caagtgcatt gttctcttgc tgaaagctcg
     4021 atggacaagt gcattgttct cttgctgaaa gctcagtacc cgggagtacc ctcgaccgcc
     4081 ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg
     4141 aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
     4201 ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca
     4261 actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat
     4321 actttcaaca agttaccgag aaagaagaac tcacacacag ctagatcaaa caagtttgta
     4381 caaaaaagca ggctccgcgg ccgccccctt caccatgggg aaaaagcaaa acaagaagaa
     4441 agtggaggag gtactagaag aagaggaaga ggaatatgtg gtggaaaaag ttcttgatcg
     4501 gcgagttgtc aagggcaagg tggaatatct tctaaagtgg aagggtttct cagatgagga
     4561 caacacttgg gagccagaag agaatctgga ttgccctgac cttattgctg agtttctaca
     4621 gtcacagaaa acagctcatg agacagataa gtcagaggga ggcaagcgca aagctgattc
     4681 tgattctgaa gataaaggag aggaaagcaa accaaagaag aagaaagaag agtcagaaaa
     4741 gccacgaggc tttgcccggg gtttggagcc agagcggatt attggagcta ctgactccag
     4801 tggagagctc atgttcctga tgaaatggaa aaactctgat gaggctgacc tggtccctgc
     4861 caaggaagcc aatgtcaagt gcccacaggt tgtcatatcc ttctatgagg aaaggctaac
     4921 gtggcattcc tacccctcag aggatgatga caaaaaagac gacaagaata agggtgggcg
     4981 cgccgaccca gctttcttgt acaaagtggt tcgatccggt ggtaagccta tccctaaccc
     5041 tctcctcggt ctcgattcta cgatcgattt cgaaccgcgg atgaagaaaa atcgcgcttt
     5101 tttgaagtgg gcagggggca agtatcccct gcttgatgat attaaacggc atttgcccaa
     5161 gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg gtgtttctca acaccgactt
     5221 ttctcgttat atccttgccg atatcaatag cgacctgatc agtctctata acattgtgaa
     5281 gatgcgtact gatgagtacg tacaggccgc acgcgagctg tttgttcccg aaacaaattg
     5341 cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa agccaggatc cgttccgtcg
     5401 ggcggtactg tttttatatt tgaaccgcta cggttacaac ggcctgtgtc gttacaatct
     5461 gcgcggtgag tttaacgtgc cgttcggccg ctacaaaaaa ccctatttcc cggaagcaga
     5521 gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc tattgtgagt cttacgccga
     5581 tagcatggcg cgcgcagatg atgcatccgt cgtctattgc gatccgcctt atgcaccgct
     5641 gtctgcgacc gccaacttta cggcgtatca cacaaacagt tttacgcttg aacaacaagc
     5701 gcatctggcg gagatcgccg aaggtctggt tgagcgccat attccagtgc tgatctccaa
     5761 tcacgatacg atgttaacgc gtgagtggta tcagcgcgca aaattgcatg tcgtcaaagt
     5821 tcgacgcagt ataagcagca acggcggcac acgtaaaaag gtggacgaac tgctggcttt
     5881 gtacaaacca ggagtcgttt cacccgcgaa aaaataactc gagacctaga aaaacatgga
     5941 gcaatcacaa gtagcaatac agcagctacc aatgctgctt gtgcctggct agaagcacaa
     6001 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6061 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6121 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6181 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     6241 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     6301 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     6361 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     6421 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6481 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6541 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6601 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6661 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6721 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6781 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6841 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6901 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6961 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7021 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     7081 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7141 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7201 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7261 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     7321 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7381 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7441 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7501 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7561 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7621 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7681 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     7741 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     7801 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     7861 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7921 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7981 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     8041 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     8101 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     8161 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8221 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8281 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8341 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8401 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8461 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8521 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8581 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8641 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8701 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8761 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8821 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8881 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8941 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9001 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9061 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9121 tgtgtgcacg aaccccccgt tcagc
//
