LOCUS       sequence-146896-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter for miRNA activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Eichelser C, Stuckrath I, Muller V, Milde-Langosch K, Wikman H, 
            Pantel K, Schwarzenbach H
  TITLE     Increased serum levels of circulating exosomal microRNA-373 in 
            receptor-negative breast cancer patients.
  JOURNAL   Oncotarget. 2014 Oct 30;5(20):9650-63.
  PUBMED    25333260
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Oncotarget."; date: "2014-10-30"; volume: "5(20)"; pages: "9650-63"
COMMENT     Sequence Label: 373_ESR1 in pmiRGlo
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(288..306)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    343..464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(354..373)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     408..427
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(732..752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
       61 ggcggcaaga tcgccgtgta attctagttg tttaaacgag ctcgctagaa gtgcaaaatt
      121 gaagtgccat aatagaggca gcaattactt agaaatttaa aaagtgctta tcagaggcaa
      181 aatgtctact ctccaggcct gggtctctag ctagagtcga cctgcaggca tgcaagctga
      241 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      301 actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt
      361 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      421 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      481 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
      541 tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
      601 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc
      661 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
      721 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      841 ctccccagca ggcagaagt
//
