LOCUS       sequence-146900-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(72..147)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(168..473)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(270..289)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctc gaggtcgaga
       61 attcaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
      121 tgaacttgct atttctagct ctcaaacggg tcttcgtcct gcagggttta tattccccag
      181 aacatcaggt taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct
      241 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      301 tccccgatat gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca
      361 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc
      421 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
      481 gtccctgttc tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat
      541 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
      601 ggttgtgctt accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
      661 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac acctcttttt gacatacttc
      721 gggtatacat atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact
      781 gttatctggc ttttagtaag cctcgaagac accggtgttt cgtcctttcc acaagatata
      841 taaagcc
//
