LOCUS       sequence-146903-         542 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the N-terminal fragment of manduca sexta soluble guanylyl 
            cyclase subunits alpha and beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Fritz BG, Roberts SA, Ahmed A, Breci L, Li W, Weichsel A, Brailey 
            JL, Wysocki VH, Tama F, Montfort WR
  TITLE     Molecular model of a soluble guanylyl cyclase fragment determined by
            small-angle X-ray scattering and chemical cross-linking.
  JOURNAL   Biochemistry. 2013 Mar 5;52(9):1568-82. doi: 10.1021/bi301570m. Epub
            2013 Feb 15.
  PUBMED    23363317
REFERENCE   2  (bases 1 to 542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi301570m";
            journalName: "Biochemistry"; date: "2013-03-5- 5"; volume: "52"; 
            issue: "9"; pages: "1568-82"
COMMENT     Sequence Label: pETDuet-msGC-NT13
FEATURES             Location/Qualifiers
     source          1..542
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    53..77
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             92..114
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agaggatcga gatcgatctc gatcccgcga aattnatacg actcactata ggggaattgt
       61 gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc
      121 atgggcagca gccatcacca tcatcaccac agccaggatc cgctcactct taagcatatg
      181 agtgaggctt tgcaactcct gactgctcca tcgaatgaat gcctccatgc agctgtgacg
      241 tcactcacga agaatcaatc agatcactac cataaataca attgtttgcg tagattacca
      301 gacgacgtaa aaacttgtag aaattacgcg tacctacaag aaatttacga cgccgtgcga
      361 gcaactgaca gtgtcaacac gaaggatttt atggcaaaat taggggaata tttaatttta
      421 acagccttct cgcacaattg tcggttagag agagcgttta aatgtttagg cacaaattta
      481 acagaatttc tgaccacact agacagtgtt catgacgtgt tgcacgatca ggacacaccg
      541 ct
//
