LOCUS       sequence-146911-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPRainbow-DONOR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pCRISPRainbow-DONOR1
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     197..216
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(336..342,343..360,361..366))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     572..591
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
       61 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
      121 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
      181 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
      241 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
      301 tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag
      361 tgtaaagcct ggggtgccta atgcggccgc ggtctctgtc aacgcgtata tctggcccgt
      421 acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgga attgtcggtc
      481 aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca
      541 caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg
      601 caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa
      661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      721 tgagacaata accctgataa atgcttcaat aatattga
//
