LOCUS       sequence-146915-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA1-2XboxB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
  TITLE     Multiplexed labeling of genomic loci with dCas9 and engineered 
            sgRNAs using CRISPRainbow.
  JOURNAL   Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
  PUBMED    27088723
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526."
COMMENT     Sequence Label: pLH-sgRNA1-2XboxB
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             305..610
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     489..508
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    643..661
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    698..716
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
       61 gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca gtgacagttg acagcgacag
      121 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
      181 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
      241 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa cagggactgg
      301 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
      361 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
      421 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
      481 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
      541 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
      601 gggaatataa accctgcagg acgaagaccc gtttgagagc tagggccctg aagaagggcc
      661 ctagcaagtt caaataaggc tagtccgtta tcaacttggg ccctgaagaa gggcccaagt
      721 ggcaccgagt cggtgctttt tttgaattct cgacctcgag acaaatggca gtattcatc
//
