LOCUS       sequence-146920-        9073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of human ESRRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-hESRRB
FEATURES             Location/Qualifiers
     source          1..9073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        383..658
                     /label=chicken beta-actin promoter
     intron          659..1667
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1591..1613
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    1745..1769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3304..3328)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3349..3365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3373..3389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3397..3403,3404..3421,3422..3427))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3442..3463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3523..3540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3529..3753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3830..3863
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4086..4105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4087..4105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4115..4132)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4115..4131)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4124..4146)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4273..4728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4360..4379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4570..4591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4754..4858
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4859..4927,4928..5719)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5077..5096)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5890..6478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6379..6398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6632..6649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6766..6787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6802..6807,6808..6825,6826..6832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6840..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6864..6880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6899..6919
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6901..6919
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   6942..6976
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(7459..8484)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7459..8484)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8503..9002)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     8944..8967
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    9025..9058
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc
      421 tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg
      481 atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg
      721 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
      781 ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct
      841 taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt
      901 gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg
      961 gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag
     1441 cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1501 gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg
     1561 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1621 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1681 tgctggttgt tgtgctgtct catcattttg gcaaagaatt cctcgacggt atcgataagc
     1741 tatcacaagt ttgtacaaaa aagcaggctc tcaccatgtc ctcggacgac aggcacctgg
     1801 gctccagctg cggctccttc atcaagactg agccgtccag cccgtcctcg ggcatcgatg
     1861 ccctcagcca ccacagcccc agtggctcgt ccgacgccag cggcggcttt ggcctggccc
     1921 tgggcaccca cgccaacggt ctggactcgc cacccatgtt tgcaggcgcc gggctgggag
     1981 gcaccccatg ccgcaagagc tacgaggact gtgccagcgg catcatggag gactcggcca
     2041 tcaagtgcga gtacatgctc aacgccatcc ccaagcgcct gtgcctcgtg tgcggggaca
     2101 ttgcctctgg ctaccactac ggcgtggcct cctgcgaggc ttgcaaggcc ttcttcaaga
     2161 ggactatcca agggaacatt gagtacagct gcccggccac caacgagtgc gagatcacca
     2221 aacggaggcg caagtcctgc caggcctgcc gcttcatgaa atgcctcaaa gtggggatgc
     2281 tgaaggaagg tgtgcgcctt gatcgagtgc gtggaggccg tcagaaatac aagcgacggc
     2341 tggactcaga gagcagccca tacctgagct tacaaatttc tccacctgct aaaaagccat
     2401 tgaccaagat tgtctcatac ctactggtgg ctgagccgga caagctctat gccatgcctc
     2461 cccctggtat gcctgagggg gacatcaagg ccctgaccac tctctgtgac ctggcagacc
     2521 gagagcttgt ggtcatcatt ggctgggcca agcacatccc aggcttctca agcctctccc
     2581 tgggggacca gatgagcctg ctgcagagtg cctggatgga aatcctcatc ctgggcatcg
     2641 tgtaccgctc gctgccctat gacgacaagc tggtgtacgc tgaggactac atcatggatg
     2701 aggagcactc ccgcctcgcg gggctgctgg agctctaccg ggccatcctg cagctggtac
     2761 gcaggtacaa gaagctcaag gtggagaagg aggagtttgt gacgctcaag gccctggccc
     2821 tcgccaactc cgattccatg tacatcgagg atctagaggc tgtccagaag ctgcaggacc
     2881 tgctgcacga ggcactgcag gactacgagc tgagccagcg ccatgaggag tcctggagga
     2941 cgggcaagct gctgctgaca ctgccgctgc tgcggcagac ggccgccaag gccgtgcagc
     3001 acttctatag cgtcaaactg cagggcaaag tgcccatgca caaactcttc ctggagatgc
     3061 tggaggccaa ggttggccaa gagcagctta gaggatctcc caaggatgaa agaatgtcaa
     3121 gccatgatgg aaaatgcccc ttccaatcag ctgccttcac aagcagggat cagagcaact
     3181 ccccggggat ccccaatcca cgcccttcta gtccaacccc cctcaatgag agaggcaggc
     3241 agatctcacc cagcactagg acaccaggag gccagggaaa gcatctctgg ctcaccatgt
     3301 aatacccagc tttcttgtac aaagtggtga tagcttggcg taatcatggt catagctgtt
     3361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3481 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc agcctcgact gtgccttcta
     3541 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3601 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3661 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3721 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3781 gctcgagatc cactagttct agcctcgagg ctagagcggc cgcgtcgaga taacttcgta
     3841 taatgtatgc tatacgaagt tatgttttgt tactttatag aagaaatttt gagtttttgt
     3901 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     3961 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacg tcaattttac
     4021 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta aagagctcca
     4081 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     4141 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4201 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4261 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4321 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4381 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4441 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4501 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4561 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4621 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4681 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     4741 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4801 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4861 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4921 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4981 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5041 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5101 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5161 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5221 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5281 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5341 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5401 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5461 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5521 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5581 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5641 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5701 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5761 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5821 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5881 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5941 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6001 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6061 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6121 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6181 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6241 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6301 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6361 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6421 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6481 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6541 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6601 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6661 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     6721 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     6781 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     6841 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     6901 aattaaccct cactaaaggg aacaaaagct gggtacctta accctagaaa gatagtctgc
     6961 gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg
     7021 tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca
     7081 atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga
     7141 ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt
     7201 tctacttacg tgataactta ttatatatat attttcttgt tatagatatc caactatgaa
     7261 acattatcat aaagcaatag taaagagcct tcagtaaaga gcaggcattt atctaatccc
     7321 accccacccc cacccccgta gctccaatcc ttccattcaa aatgtaggta ctctgttctc
     7381 acccttctta acaaagtatg acaggaaaaa acttccatgt ggacatcttt attgtttaat
     7441 agatcatcaa tttctcgact attcctttgc cctcggacga gtgctggggc gtcggtttcc
     7501 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     7561 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     7621 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     7681 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
     7741 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     7801 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     7861 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     7921 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     7981 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     8041 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     8101 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     8161 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg agatgcaata
     8221 ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     8281 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     8341 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     8401 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     8461 cgcggtgagt tcaggctttt tcatggtggc ggctggatcg gtcgaaaggc ccggagatga
     8521 ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcgagaat gccgggcctc
     8581 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     8641 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     8701 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
     8761 gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
     8821 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
     8881 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
     8941 tgccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac
     9001 ccggtagaat tcatcgatga tatcataact tcgtataatg tatgctatac gaagttatgc
     9061 ggccctcgag gtc
//
