LOCUS       sequence-146927-        8988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of human KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 8988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-hKLF4
FEATURES             Location/Qualifiers
     source          1..8988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1430..1454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1475..1491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1499..1515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1523..1529,1530..1547,1548..1553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1568..1589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1649..1666)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1655..1879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1956..1989
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2212..2231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2213..2231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2241..2258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2241..2257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2250..2272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2399..2854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2486..2505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2696..2717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2880..2984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2985..3053,3054..3845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3203..3222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4016..4604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4505..4524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4758..4775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4892..4913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4928..4933,4934..4951,4952..4958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4966..4982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4990..5006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5025..5045
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5027..5045
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   5068..5102
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(5585..6610)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5585..6610)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6629..7128)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     7070..7093
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    7151..7184
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        7200..7579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7582..7857
                     /label=chicken beta-actin promoter
     intron          7858..8866
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8790..8812
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    8944..8968
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggctgtca gtgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga
       61 agggagaaga cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc
      121 cacatgaagc gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc
      181 gtggccacag acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg
      241 cccctacctc ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc
      301 tccaattcgc tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca
      361 gcctcctctt cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc
      421 ttcacctatc cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga
      481 ggcctcctct atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac
      541 atcaacgacg tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac
      601 ccggtgtaca ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc
      661 gtgctgaagg cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc
      721 gtcagcaaag gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg
      781 ccgccgcgca cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc
      841 gctggacccc ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg
      901 cagctcccca gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac
      961 tgtcaccctg ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca
     1021 tccttcctgc ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg
     1081 ccacccggtt cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc
     1141 cggaaaagga ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag
     1201 agttcccatc tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac
     1261 tgggacggct gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa
     1321 cacacggggc accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac
     1381 cacctcgcct tacacatgaa gagacatttt taaaagggtg ggcgcgccga cccagctttc
     1441 ttgtacaaag tggtgatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1501 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1561 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1621 cgggaaacct gtcgtgccag cggatcagcc tcgactgtgc cttctagttg ccagccatct
     1681 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     1741 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     1801 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     1861 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagatccact
     1921 agttctagcc tcgaggctag agcggccgcg tcgagataac ttcgtataat gtatgctata
     1981 cgaagttatg ttttgttact ttatagaaga aattttgagt ttttgttttt ttttaataaa
     2041 taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt gtaaatataa
     2101 taaaacttaa tatctattca aattaataaa taaacgtcaa ttttacgcat gattatcttt
     2161 aacgtacgtc acaatatgat tatctttcta gggttaaaga gctccaattc gccctatagt
     2221 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2281 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2341 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2401 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2461 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2521 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2581 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2641 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2701 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2761 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2821 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2881 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2941 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3001 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3061 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3121 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3181 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3241 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3301 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3361 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3421 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3481 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3541 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3601 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3661 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3721 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3781 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3841 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3901 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3961 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4141 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4681 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4741 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4801 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4861 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4921 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4981 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     5041 aaagggaaca aaagctgggt accttaaccc tagaaagata gtctgcgtaa aattgacgca
     5101 tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc tgtgcattta
     5161 ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc
     5221 actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat cttttacgtg
     5281 acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta cttacgtgat
     5341 aacttattat atatatattt tcttgttata gatatccaac tatgaaacat tatcataaag
     5401 caatagtaaa gagccttcag taaagagcag gcatttatct aatcccaccc cacccccacc
     5461 cccgtagctc caatccttcc attcaaaatg taggtactct gttctcaccc ttcttaacaa
     5521 agtatgacag gaaaaaactt ccatgtggac atctttattg tttaatagat catcaatttc
     5581 tcgactattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     5641 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     5701 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     5761 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     5821 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     5881 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     5941 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     6001 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     6061 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     6121 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     6181 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     6241 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     6301 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     6361 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     6421 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     6481 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     6541 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     6601 gctttttcat ggtggcggct ggatcggtcg aaaggcccgg agatgaggaa gaggagaaca
     6661 gcgcggcaga cgtgcgcttt tgaagcgtgc gagaatgccg ggcctccgga ggaccttcgg
     6721 gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc
     6781 ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc
     6841 cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc
     6901 atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga
     6961 ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg
     7021 gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc agcggggctg
     7081 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattcat
     7141 cgatgatatc ataacttcgt ataatgtatg ctatacgaag ttatgcggcc ctcgaggtcg
     7201 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     7261 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     7321 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     7381 tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg cagtacatca
     7441 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     7501 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     7561 agtcatcgct attaccatgg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     7621 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     7681 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
     7741 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     7801 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     7861 gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg cccgccccgg
     7921 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     7981 tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg tgaaagcctt
     8041 aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg
     8101 tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg
     8161 cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc gggggcggtg
     8221 ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     8281 ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg cacccccctc
     8341 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg cgtggcgcgg
     8401 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     8461 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg gcggctgtcg
     8521 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     8581 tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     8641 gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     8701 cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc agggggacgg
     8761 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     8821 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     8881 gctggttgtt gtgctgtctc atcattttgg caaagaattc ctcgacggta tcgataagct
     8941 atcacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcacc
//
