LOCUS       sequence-146935-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with PGK -  Hygromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-destination
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(106..605)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(154..173)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     547..570
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    628..661
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttcatggt ggcggctgga tcggtcgaaa ggcccggaga
      121 tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc
      181 tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga
      241 cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct
      301 gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt
      361 gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa
      421 cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg
      481 ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca
      541 agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc
      601 tacccggtag aattcatcga tgatatcata acttcgtata atgtatgcta tacgaagtta
      661 tgc
//
