LOCUS       sequence-146943-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM100: no promoter RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      158..745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     646..665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     899..916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1033..1054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
       61 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt ttctgcgcgt
      181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      901 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      961 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     1021 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggcat ggcaagtagt
     1081 gaagacgtta tcaaagagtt catgcgtttc aaagtt
//
