LOCUS       sequence-146944-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM100: no promoter RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(78..100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(198..875)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     protein_bind    890..911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1028..1045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
       61 tcgatgagtt tttctaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      181 cggggctggc ttaattatta agcaccggtg gagtgacgac cttcagcacg ttcgtactgt
      241 tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa
      301 gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag
      361 tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc accgtcttcc
      421 gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat aaccggaccg
      481 tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc gtcttgcagg
      541 gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac acgttcccat
      601 ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg gtgtttaacg
      661 taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa cggcagcgga
      721 ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg accttcacct
      781 tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac tttgaaacgc
      841 atgaactctt tgataacgtc ttcactactt gccatgcctg gggtgcctaa tgagtgagct
      901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      961 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1081 ctcactcaa
//
