LOCUS       sequence-146953-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacteria expression of H2BK120C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Morgan MT, Haj-Yahya M, Ringel AE, Bandi P, Brik A, Wolberger C
  TITLE     Structural basis for histone H2B deubiquitination by the SAGA DUB 
            module.
  JOURNAL   Science. 2016 Feb 12;351(6274):725-8. doi: 10.1126/science.aac5681.
  PUBMED    26912860
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-02-12- 12"; volume: "351"; 
            issue: "6274"; pages: "725-8"
COMMENT     Sequence Label: H2BK120C
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             389..411
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(442..461)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(443..461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(545..564)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcagcagca gttacttggc gctggtgtaa caggtgacag ccttggtgcc ctcggacacg
       61 gcgtgtttgg ccaactcccc aggcagcagc agtcggaccg cggtctggat ctcccgggag
      121 gtgatggtgg agcgcttgtt gtaatgagcc aggcgggagg cttcccctgc gatgcgctca
      181 aacacatcgt tgacaaagga gttcatgatg ctcatggcct tggacgagat gccggtatcg
      241 gggtgcacct gcttcagcac cttgtacacg taaatggcat aactctcctt ccttgtcttc
      301 ctgcgctttt tcccgtcttt cttctgagtc ttggtcaccg ctttcttgga gcctttcttc
      361 ggggctggag cggacttggc catatatatc tccttcttaa agttaaacaa aattatttct
      421 agagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      481 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      541 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      601 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      661 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
      721 ggctgcttcc taaatgca
//
