LOCUS       sequence-146954-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with promoter pTac driving RFP expression (maxRFP = 
            BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM105: pTac RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      158..745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     646..665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
       61 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt ttctgcgcgt
      181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      841 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgca
//
