LOCUS       sequence-146956-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with promoter pTac driving RFP expression (maxRFP = 
            BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM105: pTac RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(78..100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(198..875)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        complement(join(902..908,909..924,925..930))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    951..972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1089..1106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
       61 tcgatgagtt tttctaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      181 cggggctggc ttaattatta agcaccggtg gagtgacgac cttcagcacg ttcgtactgt
      241 tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa
      301 gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag
      361 tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc accgtcttcc
      421 gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat aaccggaccg
      481 tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc gtcttgcagg
      541 gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac acgttcccat
      601 ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg gtgtttaacg
      661 taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa cggcagcgga
      721 ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg accttcacct
      781 tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac tttgaaacgc
      841 atgaactctt tgataacgtc ttcactactt gccatagaat tctgtttcct gtgtgaccac
      901 acattatacg agccgatgat taattgtcaa cagctcgcct ggggtgccta atgagtgagc
      961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gct
//
