LOCUS       sequence-146961-         447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Determine its activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Fahie K, Hu P, Swatkoski S, Cotter RJ, Zhang Y, Wolberger C
  TITLE     Side chain specificity of ADP-ribosylation by a sirtuin.
  JOURNAL   FEBS J. 2009 Dec;276(23):7159-76. doi: 
            10.1111/j.1742-4658.2009.07427.x. Epub 2009 Nov 6.
  PUBMED    19895577
REFERENCE   2  (bases 1 to 447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1742-4658.2009.07427.x"; journalName: "FEBS J"; date: 
            "2009-12"; volume: "276"; issue: "23"; pages: "7159-76"
COMMENT     Sequence Label: TbSIR2RP1
FEATURES             Location/Qualifiers
     source          1..447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="recognition and cleavage site for Genenase(TM) I,
                     a subtilisin BPN' variant engineered for increased 
                     substrate specificity (Carter and Wells, 1987)"
                     /label=Genenase(TM) I site
                     /translation="PGAAHY"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctcgggccgg gtgcggcaca ctacatcaca agtttgtaca aaaaagcagg cttcgaaaat
       61 ttatattttc agggcatgac agaaccgaag ttagcaacca cgcacgtagt gggtgaaccc
      121 accttcgaag gactggcacg tttcattgag cgaaacaaca tcaccaaaat atttgttatg
      181 gtgggcgcag ggataagcgt tgcagctgga atccccgact tccgctctcc acacaccggc
      241 ttgtacgcta aactcagtcg ctacaatctc aactcancgg aggacgcctt ctcactccct
      301 ctcttgcgtc aacaaccaag tgtgttttac aacattctga tggatatgga cctctggccc
      361 gggaagtatt gtcctacgac ggntcaccac tttatcagtc tactcgccaa gaagggcatg
      421 ttattatgct gttgtacgca gaacata
//
