LOCUS       sequence-146968-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP 
            = BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM103: 150bp Hps2 RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     223..240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    357..378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
       61 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      121 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
      181 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
      241 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      301 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      361 gtgagttagc tcactcatta ggcaccccag gccactttat tcaagtagta cataagtgct
      421 attggattta cctagcttaa cgttgacatc aactttgatt ccaacttaag atagtttttc
      481 taaaaactaa gcacttaatt ttttaaacca actggaggaa gtacatcgtg gcattaactc
      541 aaatggcaag tagtgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
      601 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
      661 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
      721 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
      781 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
      841 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
      901 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
      961 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
     1021 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
     1081 cctacatggc taaaa
//
