LOCUS       sequence-146969-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP 
            = BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM103: 150bp Hps2 RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(84..106)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(204..881)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
       61 tgatgctcga tgagtttttc taacctctga cacatgcagc tcccggagac ggtcacagct
      121 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      181 gggtgtcggg gctggcttaa ttattaagca ccggtggagt gacgaccttc agcacgttcg
      241 tactgttcaa cgatggtgta gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt
      301 ttgtaagcac ccggcagctg aaccggtttt ttagccatgt aggtggtttt aacttcagcg
      361 tcgtagtgac caccgtcttt cagtttcaga cgcattttga tttcaccttt cagagcaccg
      421 tcttccgggt acatacgttc ggtggaagct tcccaaccca tggttttttt ctgcataacc
      481 ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct
      541 tgcagggagg agtcctgggt aacggtaaca acaccaccgt cttcgaagtt cataacacgt
      601 tcccatttga aaccttccgg gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt
      661 ttaacgtaag ctttggaacc gtactggaac tgcggggaca ggatgtccca agcgaacggc
      721 agcggaccac ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct
      781 tcaccttcac cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg
      841 aaacgcatga actctttgat aacgtcttca ctacttgcca tttgagttaa tgccacgatg
      901 tacttcctcc agttggttta aaaaattaag tgcttagttt ttagaaaaac tatcttaagt
      961 tggaatcaaa gttgatgtca acgttaagct aggtaaatcc aatagcactt atgtactact
     1021 tgaataaagt ggcctgggg
//
