LOCUS       sequence-146979-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with promoter pHps2 driving RFP expression (maxRFP = 
            BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM102: pHps2 RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    353..374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      241 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      301 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      361 gttagctcac tcattaggca ccccaggcgg tttcgtggta gaccaaaaca agctggaaaa
      421 ggcactggct gaaaagctgt aatattgctg acttgaatag cgctaataat caatttatta
      481 gcgctattca ctttattcaa gtagtacata agtgctattg gatttaccta gcttaacgtt
      541 gacatcaact ttgattccaa cttaagatag tttttctaaa aactaagcac ttaatttttt
      601 aaaccaactg gaggaagtac atcgtggcat taactcaaat ggcaagtagt gaagacgtta
      661 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
      721 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
      781 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
      841 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
      901 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
      961 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1021 acttcccgtc cgacggtccg gttatgcaga aaaa
//
