LOCUS       sequence-146982-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with promoter pHps1 driving RFP expression (maxRFP = 
            BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM101: pHps1 RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(81..103)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(201..878)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
       61 tgctcgatga gtttttctaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      121 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      181 tgtcggggct ggcttaatta ttaagcaccg gtggagtgac gaccttcagc acgttcgtac
      241 tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg
      301 taagcacccg gcagctgaac cggtttttta gccatgtagg tggttttaac ttcagcgtcg
      361 tagtgaccac cgtctttcag tttcagacgc attttgattt cacctttcag agcaccgtct
      421 tccgggtaca tacgttcggt ggaagcttcc caacccatgg tttttttctg cataaccgga
      481 ccgtcggacg ggaagttggt accacgcagt ttaactttgt agatgaactc accgtcttgc
      541 agggaggagt cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc
      601 catttgaaac cttccgggaa ggacagtttc aggtagtccg ggatgtcagc cgggtgttta
      661 acgtaagctt tggaaccgta ctggaactgc ggggacagga tgtcccaagc gaacggcagc
      721 ggaccacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca
      781 ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa
      841 cgcatgaact ctttgataac gtcttcacta cttgccattt gtactgatgg ttgtgccacg
      901 atacttcctc cgcttaagtt acagggttat gatttactca ctccgtaatg atacattttt
      961 tcttgtgaat agggtaaatc ggttgaattt tagcaatatt ttgccatgat gcaccttaaa
     1021 tgcatcatta ggcagcatca taatcgcttt t
//
