LOCUS       sequence-146984-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCM66T with promoter pHps1 driving RFP expression (maxRFP = 
            BBa_E1010).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  TITLE     broad host range pcm66t
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJM101: pHps1 RFP on pCM66T
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     219..236
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    353..374
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
       61 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      121 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      181 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      241 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggcctatt cattaatgca
      301 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      361 gttagctcac tcattaggca ccccaggcac ttaaaagcga ttatgatgct gcctaatgat
      421 gcatttaagg tgcatcatgg caaaatattg ctaaaattca accgatttac cctattcaca
      481 agaaaaaatg tatcattacg gagtgagtaa atcataaccc tgtaacttaa gcggaggaag
      541 tatcgtggca caaccatcag tacaaatggc aagtagtgaa gacgttatca aagagttcat
      601 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      661 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      721 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      781 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      841 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      901 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      961 cggtccggtt atgcagaaaa a
//
