LOCUS       sequence-146988-        4633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Wischnewski F, Friese O, Pantel K, Schwarzenbach H
  TITLE     Methyl-CpG binding domain proteins and their involvement in the 
            regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene 
            promoters.
  JOURNAL   Mol Cancer Res. 2007 Jul;5(7):749-59.
  PUBMED    17634428
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res."; date: "2007-07"; volume: "5(7)"; pages: "749-59"
COMMENT     Sequence Label: MAGE-A3Pro pGL4basic
FEATURES             Location/Qualifiers
     source          1..4633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             491..2143
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2187..2308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2198..2217)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2252..2271
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2556..2573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2727..3315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2807..2826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3515..4306,4307..4375))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4480..4528
                     /note="synthetic polyadenylation signal"
     misc_feature    4542..4633
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4582..4601
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc ttccccagac
       61 cacacccctt cccccaatgc cacttcaggg ggactcagag tcagagactt ggtctgaggg
      121 gagcagaagc aatctgcaga ggatggaggt ccaggctcag ccaggcatca acttcaggac
      181 cctgagggat gaccgaaggc cccgcccaca cacccccaac tcccccgacc ccaccaggat
      241 ctacagcctc aggacccccg tcccaatcct taccccttgc cccatcacca tcttcatgct
      301 tacctccacc cccatccgat ccccatccag gcagaatcca gttccacccc tgcccggaac
      361 ccagggtagt accgttgcca ggatgtgacg ccactgactt gcgcattgga ggtcagaaga
      421 ccgcgagatt ctcgccctga gcaacgagcg acggcaagct tggcaatccg gtactgttgg
      481 taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact
      541 cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc
      601 cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt
      661 cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg
      721 gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt
      781 catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag
      841 catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct
      901 caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga
      961 ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa
     1021 cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa
     1081 cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt
     1141 ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat
     1201 cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat
     1261 ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt
     1321 gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa
     1381 gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc
     1441 gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg
     1501 ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga
     1561 caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga
     1621 caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat
     1681 catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg
     1741 gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg
     1801 gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat
     1861 cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc
     1921 cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga
     1981 gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt
     2041 gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga
     2101 gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg
     2161 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2221 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2281 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2341 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2401 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2461 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2521 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3481 gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac
     3541 cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga
     3601 tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc
     3661 cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa
     3721 gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta
     3781 gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg
     3841 tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc
     3901 gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg
     3961 ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt
     4021 ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt
     4081 cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca
     4141 acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc
     4201 ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac
     4261 ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca
     4321 agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg
     4381 tcctttttca atattattga agcatttatc agggttacta gtacgtctct caaggataag
     4441 taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca
     4501 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4561 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4621 agaacatttc tct
//
