LOCUS       sequence-146994-         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the ketosynthase FabF/KASII from the E. coli fatty acid 
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: FabF (KASII) pet28 N-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             58..75
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgaaacac caccaccacc accaccacca cggtggtctg
       61 gttccgcgtg gttcccatgg cgtgtctaag cgtcgtgtag ttgtgaccgg actgggcatg
      121 ttgtctcctg tcggcaatac cgtagagtct acctggaaag ctctgcttgc cggtcagagt
      181 ggcatcagcc taatcgacca tttcgatact agcgcctatg caacgaaatt tgctggctta
      241 gtaaaggatt ttaactgtga ggacattatc tcgcgcaaag aacagcgcaa gatggatgcc
      301 ttcattcaat atggaattgt cgctggcgtt caggccatgc aggattctgg ccttgaaata
      361 acggaagaga acgcaacccg cattggtgcc gcaattggct ccgggattgg cggcctcgga
      421 ctgatcgaag aaaaccacac atctctgatg aacggtggtc cacgtaagat cagcccattc
      481 ttcgttccgt caacgattgt gaacatggtg gcaggtcatc tgactatcat gtatggcctg
      541 cgtggcccga gcatctctat cgcgactgcc tgtacttccg gcgtgcacaa cattggccat
      601 gctgcgcgta ttatcgcgta tggcgatgct gacgtgatgg ttgcaggtgg cgca
//
