LOCUS       sequence-147000-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pp32(1-149) cloned into pProEX HTb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Huyton T, Wolberger C
  TITLE     The crystal structure of the tumor suppressor protein pp32 (Anp32a):
            structural insights into Anp32 family of proteins.
  JOURNAL   Protein Sci. 2007 Jul;16(7):1308-15. Epub 2007 Jun 13.
  PUBMED    17567741
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2007-07"; volume: "16(7)"; pages: "1308-15. Epub 2007 
            Jun 13"
COMMENT     Sequence Label: pp32-Delta-CT
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(470..490)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(512..529)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(551..573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    562..578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cctcgagtta gtcatagccg tcgagatatg tgagttgcgg gaggagcttg aacacatttt
       61 ctcggtagtc gttcaggttg gttacctcgc aattgaaaag gtctaagctc ttgaggtttt
      121 ctaacttttt cagtggctct attgtgctga ggtctttaat tttgttgcca cttaaattta
      181 gatgcgtgag gttcggacac ttttctgcca atacttccag gccccctgag actctgttat
      241 cgcttagttc aagcttctta agtttgttta actttggtaa gtttgcgatt gaggtgaggc
      301 ctacgttgat tgtacttaag aattccagtt cttcaaattc atctgtgagg ccttcgagtt
      361 tgccttcatt cgaccgactg ttgtccagga caagttcttt cacatcagag ggcgtcctgt
      421 tccgcagctc taaatgaatc cgtctgccca tctccatgga tcccatggcg ccctgaaaat
      481 acaggttttc cgtcgttggg atatcgtaat cgtgatggtg atggtgatgg tagtacgaca
      541 tggtctgttt cctgtgtgaa attgttatcc gctcacaatt ccacagatta tacggaccgg
      601 atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc
      661 aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac
      721 gacctgcaca gccataccac agcttccgat g
//
