LOCUS       sequence-147012-         475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Kim H, Kim ST, Ryu J, Choi MK, Kweon J, Kang BC, Ahn HM, Bae S, Kim 
            JS, Kim SG
  TITLE     A simple, flexible and high-throughput cloning system for plant 
            genome editing via CRISPR-Cas system.
  JOURNAL   J Integr Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474.
  PUBMED    26946469
REFERENCE   2  (bases 1 to 475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Integr 
            Plant Biol. 2016 Mar 4. doi: 10.1111/jipb.12474."
COMMENT     Sequence Label: pHAtC
FEATURES             Location/Qualifiers
     source          1..475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             225..245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             246..272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             246..272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(277..301)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 acaagcccat ccgcgagcag gccgagaaca tcatccacct gttcaccctg accaacctgg
       61 gcgcccccgc cgccttcaag tacttcgaca ccaccatcga ccgcaagcgc tacaccagca
      121 ccaaggaggt gctggacgcc accctgatcc accagagcat caccggtctg tacgagaccc
      181 gcatcgacct gagccagctg ggcggcgacg gcggctccgg acctccaaag aaaaagagaa
      241 aagtataccc ctacgacgtg cccgactacg cctaacaccc agctttcttg tacaaagtgg
      301 tgatatcccg cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
      361 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
      421 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttg
//
