LOCUS       sequence-147019-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses Cre-dependent 
            membrane-localized AcGFP under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell 
            EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel 
            J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G
  TITLE     Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions 
            that Are Relayed to the Ventral Tegmental Area to Regulate Learning.
  JOURNAL   Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 
            10.1016/j.cub.2017.06.024. Epub 2017 Jul 6.
  PUBMED    28690111
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2017.06.024"; journalName: "Curr Biol"; date: 
            "2017-07-24- 24"; volume: "27"; issue: "14"; pages: "2089-2100.e5"
COMMENT     Sequence Label: pOTTC710 - pAAV EF1a DIO Mem-AcGFP
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(32..65)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(116..149)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(189..902)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="SKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGN
                     YKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGF
                     VTAAAITHGMDELYK"
     protein_bind    985..1018
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 aggtaccgga tcctctagag tcgactccgg aataacttcg tataggatac tttatacgaa
       61 gttatgcaga atggtagctg gattgtagct gctattagca atatgaaacc tcttaataac
      121 ttcgtatagc atacattata cgaagttatg gcgcgcctgg ctgattatga tctagagtcg
      181 cggccgcttc acttgtacag ctcatccatg ccgtgggtga tggcggcggc ggtcacgaag
      241 ccgaagtaga tcatgtgatc gcgcttctcg ttggggtcct tggacagggc gctctgggtg
      301 gacaggtagt ggttatcggg cagcagcaca gggccatcgc cgatgggggt attctgctgg
      361 tagtggtcgg ccagctgcac gctgccatcc tcgatgttgt ggcggatctt gaagttcacc
      421 ttgatgccat tcttggcctt gtcggtcatg atgtacacat tgtgggcgtt gtagttgtac
      481 tccatcttat tgcccaggat gttgccatcc tccttgaaat cggtgccggt cagctcgatg
      541 cgattcacca gggtatcgcc ctcgaacttc acctcggcgc gcgacttgta gttgccgtca
      601 tcctcgaaga agatggtgcg ctcctggatg tagccctcag gcatggcgct cttgaagaag
      661 tcgtgctgct tcatgtgatc ggggtagcgt gagaagcact gcacgccgta gctcagggtg
      721 gtcaccaggg tgggccaggg cacaggcagc ttgccggtgg tgcagatgaa cttcagggtc
      781 agcttgccgt aggtggcatc gccctcgccc tcgccgctca cgctgaactt gtggccattc
      841 acatcgccat tcagctcgat caggatgggc acgatgccgg tgaacagctc ggcgcccttg
      901 ctgatctttt ggtcctcatc attcttttca acctgtttgg ttcttctcat acagcacagc
      961 atggtggcga ccggtgggct agcgataact tcgtataaag tatcctatac gaagttattt
     1021 gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagaat aacttcg
//
