LOCUS       sequence-147020-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses Cre-dependent 
            membrane-localized AcGFP under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell 
            EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel 
            J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G
  TITLE     Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions 
            that Are Relayed to the Ventral Tegmental Area to Regulate Learning.
  JOURNAL   Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 
            10.1016/j.cub.2017.06.024. Epub 2017 Jul 6.
  PUBMED    28690111
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2017.06.024"; journalName: "Curr Biol"; date: 
            "2017-07-24- 24"; volume: "27"; issue: "14"; pages: "2089-2100.e5"
COMMENT     Sequence Label: pOTTC710 - pAAV EF1a DIO Mem-AcGFP
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             247..960
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="SKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDGN
                     YKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFGF
                     VTAAAITHGMDELYK"
     protein_bind    1000..1033
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatcgctag cccaccggtc
      181 gccaccatgc tgtgctgtat gagaagaacc aaacaggttg aaaagaatga tgaggaccaa
      241 aagatcagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat cgagctgaat
      301 ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga tgccacctac
      361 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc ctggcccacc
      421 ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga tcacatgaag
      481 cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg caccatcttc
      541 ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg cgataccctg
      601 gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat cctgggcaat
      661 aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa ggccaagaat
      721 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt gcagctggcc
      781 gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc cgataaccac
      841 tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga tcacatgatc
      901 tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct gtacaagtga
      961 agcggccgcg actctagatc ataatcagcc aggcgcgcca taacttcgta taatgtatgc
     1021 tatacgaagt tattaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc
     1081 tgcataactt cgtataaagt atccta
//
