LOCUS       sequence-147022-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding inducible shBrd9_1061 
            (targeting murine Brd9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRE...
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(51..69,70..92,93..111,112..134,135..153,154..176,
                     177..195,196..218,219..237,238..260,261..279,280..302,
                     303..321)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        354..392
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     389..413
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cggaattgaa gatctggggg atcgatcctc tagagcatcg agtttaccac tccctatcag
       61 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      121 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      181 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      241 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      301 actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc
      361 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
      421 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctctcga
      481 cggatccacc atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg
      541 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
      601 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag g
//
