LOCUS       sequence-147025-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses Cre-dependent 
            nuclear-localized eYFP under control of the EF1a promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Sharpe MJ, Marchant NJ, Whitaker LR, Richie CT, Zhang YJ, Campbell 
            EJ, Koivula PP, Necarsulmer JC, Mejias-Aponte C, Morales M, Pickel 
            J, Smith JC, Niv Y, Shaham Y, Harvey BK, Schoenbaum G
  TITLE     Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions 
            that Are Relayed to the Ventral Tegmental Area to Regulate Learning.
  JOURNAL   Curr Biol. 2017 Jul 24;27(14):2089-2100.e5. doi: 
            10.1016/j.cub.2017.06.024. Epub 2017 Jul 6.
  PUBMED    28690111
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2017.06.024"; journalName: "Curr Biol"; date: 
            "2017-07-24- 24"; volume: "27"; issue: "14"; pages: "2089-2100.e5"
COMMENT     Sequence Label: pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(159..179)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(183..203)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(207..227)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(252..962,963..965,966..968))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     643..662
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cctctagagt cgactccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa
       61 tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca
      121 tacattatac gaagttatgg cgcgccgtcg acctatcata cctttctctt cttttttgga
      181 tctaccttgc gcttcttctt ggggtctacc ttgcgcttct tttttgggtc ggcgcggctg
      241 cgcaggccgg acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      301 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag
      361 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      421 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      481 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      541 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      601 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      661 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      721 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg
      781 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      841 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      901 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      961 ctcaccat
//
