LOCUS       sequence-147048-         643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from LentiCRISPR v1 but expresses tagBFP instead of 
            puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 643)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLentiCRISPR-tagBFP
FEATURES             Location/Qualifiers
     source          1..643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..94
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tggtcaagct aagaaaaaga aagctagcgg cagcggcgca accaacttca gcctgctgaa
       61 gcaggccggc gacgtggagg agaaccccgg ccccagcgag ctgattaagg agaacatgca
      121 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
      181 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      241 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
      301 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
      361 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      421 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
      481 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
      541 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
      601 gatcgcaaac atcaagacca catatagatc caagaaaccc gct
//
