LOCUS       sequence-147051-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from LentiCRISPR v1 but expresses RFP657 instead of 
            puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLentiCRISPR-RFP657
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..77
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             87..143
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggatcgacct gtctcagctg ggaggcgaca agcgtcctgc tgctactaag aaagctggtc
       61 aagctaagaa aaagaaagct agcggcgcaa caaacttctc tctgctgaaa caagccggag
      121 atgtcgaaga gaatcctgga ccgagcgagc tgattaagga gaacatgcac atgaagctgt
      181 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
      241 cctacgaggg cacccagacc cagagaatca aggtggtcga gggcggccct ctccccttcg
      301 ccttcgacat cctggctacc agcttcatgt acggcagtca caccttcatc aaccacaccc
      361 agggcatccc cgacttctgg aagcagtcct tccctgaggg cttcacatgg gagagagtca
      421 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
      481 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc
      541 agaagaaaac actcggctgg gaggcccaca ccgagatgct gtaccccgct gacggcggcc
      601 tggaaggcag aaccgcgctg gccctgaagc tcgtgggcgg gggccacctg atctgcaact
      661 tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
      721 atgtggacta cagactggaa agaatcaag
//
