LOCUS       sequence-147052-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Derived from LentiCRISPR v1 but expresses RFP657 instead of 
            puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLentiCRISPR-RFP657
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..737)
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVV
                     EGGPLPFAFDILATSFMYGSHTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEAHTEMLYPADGGLEGRTALALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDYRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(738..794)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(804..851)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgttt aattaagctt gtgccccagt
       61 ttgctaggga ggtcgcagta tctggccaca gccacctcgt gctgctcgac gtaggtctct
      121 ttgtcggcct ccttgattct ttccagtctg tagtccacat agtagacgcc gggcatcttg
      181 aggttcttag cgggtttctt ggatctgtat gtggtcttga agttgcagat caggtggccc
      241 ccgcccacga gcttcagggc cagcgcggtt ctgccttcca ggccgccgtc agcggggtac
      301 agcatctcgg tgtgggcctc ccagccgagt gttttcttct gcatcacagg gccgttggat
      361 gggaagttca cccctctgat cttgacgttg tagatgaggc agccgtcctg gaggctggtg
      421 tcctgggtag cggtcagcac gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag
      481 ccctcaggga aggactgctt ccagaagtcg gggatgccct gggtgtggtt gatgaaggtg
      541 tgactgccgt acatgaagct ggtagccagg atgtcgaagg cgaaggggag agggccgccc
      601 tcgaccacct tgattctctg ggtctgggtg ccctcgtagg gcttgccttc gccctcggat
      661 gtgcacttga agtggtggtt gttcacggtg ccctccatgt acagcttcat gtgcatgttc
      721 tccttaatca gctcgctcgg tccaggattc tcttcgacat ctccggcttg tttcagcaga
      781 gagaagtttg ttgcgccgct agctttcttt ttcttagctt gaccagcttt cttagtagca
      841 gcaggacgct tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg
      901 atgctctggt ggatca
//
