LOCUS       sequence-147055-       10208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID lentiviral vector for fusing gene of interest with 
            Dam-V5 using Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pLgw RFC1-V5-EcoDam
FEATURES             Location/Qualifiers
     source          1..10208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      266..694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(353..372)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     563..584
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(703..723)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        708..1038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      889..1024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     951..970
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1086..1133
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1152..1526
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1656..1777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1693..1712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1747..1766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1826..1842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1826..1842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1839..1861)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1850..1866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1874..1880,1881..1898,1899..1904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1919..1940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2057..2074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2228..2816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2308..2327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2987..3778,3779..3847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3610..3629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3848..3952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4027..4046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4218..4597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4599..4797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4752..4772
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4815..4995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5039..5164
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5657..5890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6075..6119
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6581..6819
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6766..6787
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6872..6996
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7021..7026,7027..7044,7045..7051)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7105..7785
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(7171..7190)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8105..8410
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8289..8308
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8451..8575)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8586..8627
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     LTR             9992..10172
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(join(10198..10208,1..7))
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(10204..10208,1..220)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
       61 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      121 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      181 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      241 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
      301 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
      361 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
      421 ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
      481 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
      541 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
      601 atctcggtct attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa
      661 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
      721 ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat
      781 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
      841 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
      901 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
      961 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     1021 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     1081 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     1141 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     1201 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     1261 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     1321 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     1381 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     1441 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     1501 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     1561 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     1621 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     1681 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     1801 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1861 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1921 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1981 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2041 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2101 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2161 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2221 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2281 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2341 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2401 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     2461 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2521 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2581 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2641 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2701 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2761 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2821 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2881 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2941 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3001 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3061 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3121 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3181 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3241 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3301 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3361 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3421 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3481 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3541 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3601 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3661 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3721 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3781 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3841 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3901 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3961 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac
     4021 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     4081 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     4141 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     4201 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     4261 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4321 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4381 agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc
     4441 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4501 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4561 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     4621 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4681 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     4741 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     4801 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4981 ggaaaatctc tagcagtggc gcccgaacag ggacctgaaa gcgaaaggga aaccagagct
     5041 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     5101 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     5161 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     5221 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     5281 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     5341 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     5401 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     5461 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     5521 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     5581 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     5641 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     5701 tgggcgcagc ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     5761 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     5821 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     5881 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     5941 ggaatgctag ttggagtaat aaatctctgg aacagatctg gaatcacacg acctggatgg
     6001 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6061 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     6121 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     6181 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     6241 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     6301 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     6361 acggatcccc cgggctgcag gaattcagat ctcggccgca tattaagtgc attgttctcg
     6421 ataccgctaa gtgcattgtt ctcgttagct cgatggacaa gtgcattgtt ctcttgctga
     6481 aagctcgatg gacaagtgca ttgttctctt gctgaaagct cgatggacaa gtgcattgtt
     6541 ctcttgctga aagctcagta cccgggagta ccctcgaccg ccggagtata aatagaggcg
     6601 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     6661 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
     6721 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
     6781 tgccaagaag taattattga atacaagaag agaactctga atactttcaa caagttaccg
     6841 agaaagaaga actcacacac agctagatca aacaagtttg tacaaaaaag ctgaacgaga
     6901 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     6961 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     7021 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     7081 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     7141 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     7201 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     7261 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     7321 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     7381 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     7441 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     7501 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     7561 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     7621 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     7681 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     7741 gatgagtggc aggcggggcg taatctagag gatccggctt actaaaagcc agataacagt
     7801 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     7861 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     7921 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     7981 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     8041 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     8101 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     8161 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     8221 acgtctgctg tcagataaag tcccccgtga actttacccg gtggtgcata tcggggatga
     8281 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     8341 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     8401 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     8461 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     8521 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttcgat
     8581 ccggtggtaa gcctatccct aaccctctcc tcggtctcga ttctacgatc gatttcgaac
     8641 cgcggatgaa gaaaaatcgc gcttttttga agtgggcagg gggcaagtat cccctgcttg
     8701 atgatattaa acggcatttg cccaagggcg aatgtctggt tgagcctttt gtaggtgccg
     8761 ggtcggtgtt tctcaacacc gacttttctc gttatatcct tgccgatatc aatagcgacc
     8821 tgatcagtct ctataacatt gtgaagatgc gtactgatga gtacgtacag gccgcacgcg
     8881 agctgtttgt tcccgaaaca aattgcgccg aggtttacta tcagttccgc gaagagttca
     8941 acaaaagcca ggatccgttc cgtcgggcgg tactgttttt atatttgaac cgctacggtt
     9001 acaacggcct gtgtcgttac aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca
     9061 aaaaacccta tttcccggaa gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct
     9121 ttttctattg tgagtcttac gccgatagca tggcgcgcgc agatgatgca tccgtcgtct
     9181 attgcgatcc gccttatgca ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa
     9241 acagttttac gcttgaacaa caagcgcatc tggcggagat cgccgaaggt ctggttgagc
     9301 gccatattcc agtgctgatc tccaatcacg atacgatgtt aacgcgtgag tggtatcagc
     9361 gcgcaaaatt gcatgtcgtc aaagttcgac gcagtataag cagcaacggc ggcacacgta
     9421 aaaaggtgga cgaactgctg gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaat
     9481 aactcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     9541 tgcttgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     9601 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9661 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     9721 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     9781 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     9841 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     9901 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     9961 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
    10021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10141 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
    10201 cgactgtg
//
