LOCUS       sequence-147060-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCRISPR-EGFP with sgRNA targeting human RIPk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLC-EGFP-RIP1
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(23..271)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(81..100)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(251..271)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(449..493)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    678..911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cgattcgctt cccagcagag ccggtgtttc gtcctttcca caagatatat aaagccaaga
       61 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
      121 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
      181 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
      241 aatatgaagg aatcatggga aataggccct ctctgggtcc cctcggggtt gggaggtggg
      301 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
      361 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
      421 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
      481 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
      541 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
      601 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
      661 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
      721 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
      781 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
      841 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
      901 acaaagctcc tattcccact gctcttt
//
