LOCUS       sequence-147073-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_arC9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..155)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca ttgggccagg attctcctcg
      121 acgtcaccgc atgttagcag acttcctctg ccctctccac tgccggattc ggatccgtcg
      181 cctcccagct gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc
      241 agggtggcgt ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca
      301 aagtacttga aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc
      361 tcggcctgct ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc
      421 agattagcgt cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg
      481 tccaggtagt gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag
      541 cccttcagct tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg
      601 gccagttcgt ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt
      661 tccagctcga acagggagta cttaggcagc ttgatgatca ggtcctttt
//
