LOCUS       sequence-147074-         518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCRISPR-EGFP with sgRNA targeting human RIP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 518)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLC-EGFP-RIP3
FEATURES             Location/Qualifiers
     source          1..518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    111..228
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        297..508
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
       61 tgcttttttg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
      121 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
      181 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
      241 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc
      301 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      361 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      421 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      481 tcgcaacggg tttgccgcca gaacacaggt gtcgtgac
//
