LOCUS       sequence-147075-         556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCRISPR-EGFP with sgRNA targeting human RIP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 556)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLC-EGFP-RIP3
FEATURES             Location/Qualifiers
     source          1..556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(25..273)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(83..102)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(253..273)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aacaccgaac catcactcgt gtacggtgtt tcgtcctttc cacaagatat ataaagccaa
       61 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
      121 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
      181 ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg
      241 caaatatgaa ggaatcatgg gaaataggcc ctctctgggt cccctcgggg ttgggaggtg
      301 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
      361 aaaaactatt cttaaaccta ccaagccctg atgaatcccc taatgatttt tatcaaaatc
      421 attaagttaa ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat
      481 aatcagacaa caaaaatgtg cgaactcgat attttacacg actctcttta ccaattctgc
      541 cccgaattac acttaa
//
