LOCUS       sequence-147078-         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LentiCRISPR-BFP with sgRNA targeting human FADD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   McComb S, Aguade-Gorgorio J, Harder L, Marovca B, Cario G, Eckert C,
            Schrappe M, Stanulla M, von Stackelberg A, Bourquin JP, Bornhauser 
            BC
  TITLE     Activation of concurrent apoptosis and necroptosis by SMAC mimetics 
            for the treatment of refractory and relapsed ALL.
  JOURNAL   Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 
            10.1126/scitranslmed.aad2986.
  PUBMED    27194728
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2016-05-18"; volume: "8(339):339ra70. doi"; pages: " 
            10.1126/scitranslmed.aad2986"
COMMENT     Sequence Label: pLC-BFP-FADD
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        28..103
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    151..268
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        337..548
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 aacaccgact cccgcacacg ctctgtcgtt ttagagctag aaatagcaag ttaaaataag
       61 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattctagat
      121 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      181 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      241 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
      301 ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      361 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      421 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      481 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      541 gaacacaggt gtcgtgacgc gggatc
//
